Evaluation of the Vysis IntelliFISH Hybridization Buffer and Vysis IntelliFISH Universal FFPE Pretreatment and Wash Kit in FISH Assays 【Abstract】

[N A J Med Sci. 2017;10(1):5-7.   DOI:  10.7156/najms.2017.1001005]

Jiawei Zhao, MS; Chengwen Li, BS; Jing Xiao, MS; Yingchun Zheng, MS; Kejing Tang, BS; Shenghua Chen, MD; Kun Ru, MD, PhD

Standard fluorescence in-situ hybridization (FISH) technique requires a hybridization time of 12 hours or greater, thus amounting to a turn-around time (TAT) of 24 hours or greater for result reporting. To improve the TAT for the FISH tests, a new Vysis IntelliFISH Hybridization Buffer has been recently developed by Abbott Molecular. It was evaluated in 20 pairs of matched bone marrow samples and 10 pairs of matched formalin-fixed lymphoma tissue samples against the standard Vysis LSI/WCP Hybridization Buffer protocol. Compared to the standard Vysis LSI/WCP Hybridization Buffer protocol, the new fast working Vysis IntelliFISH Hybridization Buffer protocol reduced FISH hybridization time and simplified the workflow of the standard overnight hybridization protocol. In addition, signal intensity, slide background and signal specificity of FISH probes were comparable to those generated with the standard hybridization protocol.

Key Words: fluorescence in situ hybridization assay, leukemia, lymphoma

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Jiawei Zhao, MS;* Chengwen Li, BS; Jing Xiao, MS; Yingchun Zheng, MS; Kejing Tang, BS; Shenghua Chen, MD; Kun Ru, MD, PhD
Department of Pathology and Laboratory Medicine, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences

*Corresponding Author: Department of Pathology and Laboratory Medicine, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences, Tianjin 300020, China. Tel: (86)-022-23909368. (Email: rukun@ihcams.ac.cn)

CONFLICT OF INTEREST
None.

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